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Showing 1–8 of 8 results
Advanced filters: Author: G. Brett Robb Clear advanced filters

  • A method termed ac4C-seq is introduced for the transcriptome-wide mapping of the RNA modification N4-acetylcytidine, revealing widespread temperature-dependent acetylation that facilitates thermoadaptation in hyperthermophilic archaea.

    • Aldema Sas-Chen
    • Justin M. Thomas
    • Schraga Schwartz
    Research
    Nature
    Volume: 583, P: 638-643

  • The epitranscriptome is fitness-relevant across Domains. Here, the authors map m5C in the model hyperthermophile, Thermococcus kodakarensis. The abundance and complexity of the m5C epitranscriptome in T. kodakarensis argues that m5C supports life in the extremes.

    • Kristin A. Fluke
    • Ryan T. Fuchs
    • Thomas J. Santangelo
    ResearchOpen Access
    Nature Communications
    Volume: 15, P: 1-20

  • A few bacterial Cas9 nucleases have been repurposed as genome editing tools. Here, the authors use bioinformatic and biochemical analyses to characterize 79 Cas9 proteins, revealing substantial functional diversity and thus expanding the available toolbox of RNA-programmable CRISPR-associated nucleases.

    • Giedrius Gasiunas
    • Joshua K. Young
    • Virginijus Siksnys
    ResearchOpen Access
    Nature Communications
    Volume: 11, P: 1-10

  • A PAMless CRISPR nuclease is applied for precision DNA cleavage and cloning.

    • Kathleen A. Christie
    • Jimmy A. Guo
    • Benjamin P. Kleinstiver
    Research
    Nature Biotechnology
    Volume: 41, P: 409-416

  • Songbirds originated in Australia and have now diversified into approximately 5,000 species found across the world. Here, Moyle et al. combine phylogenomic and biogeographic analyses to show that songbird diversification was associated with the formation of islands providing a route out of Australia.

    • Robert G. Moyle
    • Carl H. Oliveros
    • Brant C. Faircloth
    ResearchOpen Access
    Nature Communications
    Volume: 7, P: 1-7

  • Two distinct Cas12a orthologs (Cme and Yme) from metagenomic sources are characterized. The enzymes are found to be thermotolerant and are compared to three mesophilic Cas12a enzymes (Lba, Fno and Asp).

    • Ryan T. Fuchs
    • Jennifer L. Curcuru
    • G. Brett Robb
    ResearchOpen Access
    Communications Biology
    Volume: 5, P: 1-16