Nanopore direct RNA sequencing is promising for epitranscriptome study but remains limited in single-molecule resolution. Here, the authors develop SingleMod, a tool for accurate single-molecule m6A detection from DRS and reveal cross-species epitranscriptome landscapes at single-molecule level.
- Ying-Yuan Xie
- Zhen-Dong Zhong
- Guan-Zheng Luo
