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Showing 1–5 of 5 results
Advanced filters: Author: Jonatan Alvelid Clear advanced filters

  • Monomeric near-infrared (NIR) fluorescent proteins (FPs) from bacterial phytochromes bring potential advantages, but their brightness in cells is lower than dimeric NIR FPs. Here the authors develop enhanced monomeric NIR FPs enabling imaging across different scales without the trade-off between brightness and monomeric state.

    • Mikhail E. Matlashov
    • Daria M. Shcherbakova
    • Vladislav V. Verkhusha
    ResearchOpen Access
    Nature Communications
    Volume: 11, P: 1-12

  • Photolabeling of intracellular molecules is an invaluable approach to study multiple cellular processes. Here, the authors report on the near-infrared to far-red photoconversion in the miRFP family of fluorescent proteins, which enables photolabeling entirely performed in the near-infrared range.

    • Francesca Pennacchietti
    • Jonatan Alvelid
    • Ilaria Testa
    ResearchOpen Access
    Nature Communications
    Volume: 14, P: 1-14

  • Event-triggered STED is an automated approach that can initiate 2D or 3D STED imaging of specific regions in biological samples after detection of an event of interest. This approach can help maximize observations in live cell imaging and enable discovery.

    • Jonatan Alvelid
    • Martina Damenti
    • Ilaria Testa
    ResearchOpen Access
    Nature Methods
    Volume: 19, P: 1268-1275

  • Stimulated emission depletion microscopy opens new observation windows in challenging samples such as living cells and tissues. In this Primer, Lukinavičius et al. discuss 2D and 3D stimulated emission depletion setup, including adaptive optical elements and their combination with fluorescence lifetime techniques.

    • Gražvydas Lukinavičius
    • Jonatan Alvelid
    • Ilaria Testa
    Reviews
    Nature Reviews Methods Primers
    Volume: 4, P: 1-22