Kristoffer Weber et al. describe a new lentiviral vector-mediated RGB (red, green and blue) multicolor cell marking technique for analyzing clonal cell fates in vitro and in vivo. Here they use RGB marking to assess clonality after regeneration of injured livers by transplanted primary hepatocytes, to mark hematopoietic stem/progenitor cells and to assess the clonality of tumor cells. The approach can potentially be adapted to various cell types and other vector systems.
- Kristoffer Weber
- Michael Thomaschewski
- Boris Fehse
