Alanine substitution in peptides is crucial for studying peptide-based inhibitors of protein–protein interactions, but limited information is obtained from single alanine substitution. Here, the authors develop a label-free combinatorial alanine affinity selection platform to establish multi-alanine mutational tolerance and provide structure-activity relationships.
- Xiyun Ye
- Yen-Chun Lee
- Bradley L. Pentelute
