Human manganese superoxide dismutase is an oxidoreductase that converts superoxide to molecular oxygen and hydrogen peroxide with proton-coupled electron transfers (PCETs), and has evolved to be highly product inhibited to limit the formation of hydrogen peroxide. Here, the authors use neutron diffraction and X-ray absorption spectroscopy of the product-bound, trivalent, and divalent states of the enzyme to identify the product-inhibited complex and propose a PCET mechanism.
- Jahaun Azadmanesh
- Katelyn Slobodnik
- Gloria E. O. Borgstahl
