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Nature Microbiology (1)

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Inducible transposon mutagenesis identifies bacterial fitness determinants during infection in mice

InducTn-seq, a method for inducible mutagenesis followed by transposon insertion site sequencing, enables temporal control of transposition to bypass population bottlenecks and enable the quantification of gene fitness during in vivo Citrobacter rodentium infection.

David W. Basta
Ian W. Campbell
Matthew K. Waldor
ResearchOpen Access27 Mar 2025
Nature Microbiology

Volume: 10, P: 1171-1183

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Inducible transposon mutagenesis identifies bacterial fitness determinants during infection in mice

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