Histone methylation by histone lysine methyltransferases (HKMTs) is a vital post-translational modification regulating gene expression, however, selective mapping of methylation by proteomics analysis remains challenging. Here, the authors develop a heavy co-factor analogue 13CD3-BrSAM for HKMT DOT1L that can selectively heavy label target substrates, and map their methylation by proteomics.
- Nicole Trainor
- Harry J. Whitwell
- Matthew J. Fuchter
