Nature Search

Small molecules promote CRISPR-Cpf1-mediated genome editing in human pluripotent stem cells

Precise genome editing in human pluripotent stem cells requires the development of methods for rapid and efficient genetic manipulation. Here, the authors screen for small molecules that enhance CRISPR-Cpf1-mediated genome engineering.

Xiaojie Ma
Xi Chen
Saiyong Zhu
ResearchOpen Access03 Apr 2018
Nature Communications

Volume: 9, P: 1-7
Human pancreatic beta-like cells converted from fibroblasts

Insulin-producing pancreatic beta cells, generatedin vitro, could lead to new anti-diabetic therapies. Here, Zhu et al. convert human fibroblasts into endodermal progenitors that differentiate in vitrointo glucose-responsive beta-like cells that, following transplantation in mice, protect from diabetes.

Saiyong Zhu
Holger A. Russ
Sheng Ding
ResearchOpen Access06 Jan 2016
Nature Communications

Volume: 7, P: 1-13
A fast chemical reprogramming system promotes cell identity transition through a diapause-like state

Chen et al. develop a fast chemical reprogramming system that promotes the transition towards pluripotency through a unique diapause-like state.

Xi Chen
Yunkun Lu
Saiyong Zhu
Research07 Aug 2023
Nature Cell Biology

Volume: 25, P: 1146-1156
N⁶-methyladenosine modification-mediated mRNA metabolism is essential for human pancreatic lineage specification and islet organogenesis

Ma et al. profile the dynamic landscape of m⁶A during pancreatic differentiation, and identify ALKBH5 as an essential m⁶A regulator supporting pancreatic differentiation, indicating a role for m⁶A-mediated mRNA metabolism in cell-fate specification.

Xiaojie Ma
Jie Cao
Saiyong Zhu
ResearchOpen Access18 Jul 2022
Nature Communications

Volume: 13, P: 1-14
Construction of a human cell landscape at single-cell level

Single-cell RNA sequencing is used to generate a dataset covering all major human organs in both adult and fetal stages, enabling comparison with similar datasets for mouse tissues.

Xiaoping Han
Ziming Zhou
Guoji Guo
Research25 Mar 2020
Nature

Volume: 581, P: 303-309
Metabolic control of T_H17 and induced T_reg cell balance by an epigenetic mechanism

Metabolic changes in T cells can affect the genomic methylation status of key transcription factors and regulate the fate decision between induced regulatory T cells and T helper 17 cells.

Tao Xu
Kelly M. Stewart
Sheng Ding
Research02 Aug 2017
Nature

Volume: 548, P: 228-233
Reprogramming fibroblasts toward cardiomyocytes, neural stem cells and hepatocytes by cell activation and signaling-directed lineage conversion

A common set of reprogramming factors is used to temporarily activate somatic cells into a transition state; these cells are subsequently directed to various cell types via lineage-specific signals, circumventing the need to establish a pluripotent state.

Saiyong Zhu
Haixia Wang
Sheng Ding
Protocols04 Jun 2015
Nature Protocols

Volume: 10, P: 959-973
Mouse liver repopulation with hepatocytes generated from human fibroblasts

Human fibroblasts can be converted into hepatocytes capable of repopulating mouse livers by shortcutting reprogramming to pluripotency with factors promoting endoderm and hepatocyte differentiation.

Saiyong Zhu
Milad Rezvani
Sheng Ding
Research23 Feb 2014
Nature

Volume: 508, P: 93-97
Small molecules enable OCT4-mediated direct reprogramming into expandable human neural stem cells

Saiyong Zhu
Rajesh Ambasudhan
Sheng Ding
Correspondence03 Dec 2013
Cell Research

Volume: 24, P: 126-129

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