Nature Search

Wing Interferential Patterns (WIPs) and machine learning, a step toward automatized tsetse (Glossina spp.) identification

Arnaud Cannet
Camille Simon-Chane
Denis Sereno
ResearchOpen Access22 Nov 2022
Scientific Reports

Volume: 12, P: 1-15
Structural basis for antibiotic transport and inhibition in PepT2

The uptake and elimination of beta-lactam antibiotics are facilitated by the proton-coupled peptide transporters. Here authors present cryo-EM structures of PepT2 in complex with the cefadroxil, amoxicillin and cloxacillin to reveal a mechanism of beta-lactam antibiotic recognition

Joanne L. Parker
Justin C. Deme
Simon Newstead
ResearchOpen Access09 Oct 2024
Nature Communications

Volume: 15, P: 1-14
Crystal structures of a double-barrelled fluoride ion channel

Microorganisms can export toxic fluoride ions through highly selective channels of the Fluc family; here, the crystal structures of two bacterial Fluc homologues are presented, revealing that selectivity for small F⁻ ions may arise from the proteins’ narrow pores and unusual anion coordination.

Randy B. Stockbridge
Ludmila Kolmakova-Partensky
Simon Newstead
Research07 Sept 2015
Nature

Volume: 525, P: 548-551
Cryo-EM structure of the human THIK-1 K2P K⁺ channel reveals a lower Y gate regulated by lipids and anesthetics

In this study, Rödström et al. use cryo-EM to reveal a range of structural features in THIK-1 K⁺ channel and demonstrate how these features define many functional properties of the channel, thus expanding its potential as a drug target.

Karin E. J. Rödström
Bisher Eymsh
Stephen J. Tucker
ResearchOpen Access26 Feb 2025
Nature Structural & Molecular Biology

P: 1-8
Molecular basis for selective uptake and elimination of organic anions in the kidney by OAT1

Organic anion transporter 1 (OAT1) structures reveal the molecular basis for the selective uptake and elimination of metabolites, drugs and toxins in the kidney.

Joanne L. Parker
Takafumi Kato
Simon Newstead
ResearchOpen Access23 Jul 2023
Nature Structural & Molecular Biology

Volume: 30, P: 1786-1793
Extracellular modulation of TREK-2 activity with nanobodies provides insight into the mechanisms of K2P channel regulation

K2P channels are important regulators of cellular electrical activity. Here the authors show how nanobody fragments can be used to detect and modulate TREK2 K2P channel activity to provide insight into the mechanism of gating.

Karin E. J. Rödström
Alexander Cloake
Stephen J. Tucker
ResearchOpen Access16 May 2024
Nature Communications

Volume: 15, P: 1-13
Future opportunities in solute carrier structural biology

In this Perspective, the author describes the recent progress in understanding solute carrier (SLC) biology and discusses the roles of new families of atypical SLCs.

Simon Newstead
Reviews18 Apr 2024
Nature Structural & Molecular Biology

Volume: 31, P: 587-590
Structural basis for proton coupled cystine transport by cystinosin

Mutations in CTNS, the lysosomal cystine-proton symporter, cause cystinosis. Here authors report crystal structures of CTNS from Arabidopsis thaliana in complex with cystine, and establish the mode of ligand recognition and mechanism for proton-coupled cystine export from the lysosome.

Mark Löbel
Sacha P. Salphati
Simon Newstead
ResearchOpen Access17 Aug 2022
Nature Communications

Volume: 13, P: 1-12
Molecular basis for redox control by the human cystine/glutamate antiporter system xc⁻

System xc- is a cystine transporter that is expressed in the plasma membrane and imports cystine in exchange for intracellular glutamate. Here, the authors present the cryo-EM structure of human system xc- both in the apo form and the glutamate bound state, and further supported by molecular dynamics and cell-based assays they discuss its cystine transport mechanism.

Joanne L. Parker
Justin C. Deme
Simon Newstead
ResearchOpen Access08 Dec 2021
Nature Communications

Volume: 12, P: 1-11
Structural basis of antifolate recognition and transport by PCFT

Cryo-electron microscopy structures of PCFT in a substrate-free state and bound to the antifolate drug pemetrexed provide insights into how this protein recognizes folates and mediates their transport into cells.

Joanne L. Parker
Justin C. Deme
Simon Newstead
Research26 May 2021
Nature

Volume: 595, P: 130-134
Molecular basis of nitrate uptake by the plant nitrate transporter NRT1.1

In Arabidopsis thaliana the phosphorylation state of the ‘dual affinity’ transporter, NRT1.1, allows the uptake of nitrate over a wide concentration range; the crystal structure and molecular basis for this is described in this study.

Joanne L. Parker
Simon Newstead
Research26 Feb 2014
Nature

Volume: 507, P: 68-72
Structural basis for substrate specificity and regulation of nucleotide sugar transporters in the lipid bilayer

In eukaryotes the SLC35 family of solute carriers mediate the selective uptake of nucleotide sugars from the cytoplasm into the Endoplasmic Reticulum or Golgi. Here authors report the crystal structure of the yeast GDP-mannose transporter, Vrg4, bound to guanine monophosphate (GMP) revealing the molecular basis for GMP recognition and transport.

Joanne L. Parker
Robin A. Corey
Simon Newstead
ResearchOpen Access11 Oct 2019
Nature Communications

Volume: 10, P: 1-10
Structural basis of Smoothened regulation by its extracellular domains

Structural studies show that the activity of the G-protein-coupled receptor Smoothened is modulated by ligand-regulated interactions between its extracellular and transmembrane domains.

Eamon F. X. Byrne
Ria Sircar
Christian Siebold
Research20 Jul 2016
Nature

Volume: 535, P: 517-522
Structural basis for amino acid transport by the CAT family of SLC7 transporters

Cationic amino acid transporters (CATs) belong to the physiologically important solute carrier (SLC) 7 family. Here, the authors present the structure of the mammalian CAT transporter homologue Geobacillus kaustophilus GkApcT, which reveals how arginine is recognized, and propose a model for proton-coupled amino acid transport.

Katharina E. J. Jungnickel
Joanne L. Parker
Simon Newstead
ResearchOpen Access07 Feb 2018
Nature Communications

Volume: 9, P: 1-12
Structural basis for triacylglyceride extraction from mycobacterial inner membrane by MFS transporter Rv1410

Triacylglycerides help to secure the impermeability of mycobacterial cell envelope to some drugs. Here, authors solve the structure of the triacylglyceride transporter Rv1410 and proposed a molecular mechanism for triacylglyceride extraction.

Sille Remm
Dario De Vecchis
Markus A. Seeger
ResearchOpen Access13 Oct 2023
Nature Communications

Volume: 14, P: 1-17
Insights into L-type heteromeric amino acid transporters

Two recent cryo-EM structures of the human L-type heteromeric amino acid transporter LAT1–CD98hc reveal surprising new insights into both amino acid transport in the human body and the roles of CD98hc as a cell-surface antigen and trafficking chaperone.

Simon Newstead
News & Views03 Jun 2019
Nature Structural & Molecular Biology

Volume: 26, P: 395-396
Structural basis of nucleotide sugar transport across the Golgi membrane

Crystal structures of the nucleotide sugar transporter Vrg4 are reported in both the substrate-free and the bound states.

Joanne L. Parker
Simon Newstead
Research01 Nov 2017
Nature

Volume: 551, P: 521-524
A two-___domain elevator mechanism for sodium/proton antiport

The X-ray crystal structure of NapA, a Na⁺/H⁺ antiporter from Thermus thermophilus, in an active, outward-facing state is reported; comparisons to the structure of a related transporter in a low pH/inactivated, inward-facing state show the conformational changes that occur when the membrane protein moves from an inward-facing to an outward-facing state, suggesting that Na⁺/H⁺ antiporters operate by a two-___domain rocking bundle model.

Chiara Lee
Hae Joo Kang
David Drew
Research01 Sept 2013
Nature

Volume: 501, P: 573-577
The KDEL trafficking receptor exploits pH to tune the strength of an unusual short hydrogen bond

Zhiyi Wu
Simon Newstead
Philip C. Biggin
ResearchOpen Access09 Oct 2020
Scientific Reports

Volume: 10, P: 1-12
Overcoming barriers to membrane protein structure determination

Roslyn M Bill
Peter J F Henderson
Horst Vogel
Reviews08 Apr 2011
Nature Biotechnology

Volume: 29, P: 335-340
Generation of synthetic nanobodies against delicate proteins

This protocol describes in vitro procedures for generation of synthetic single-___domain antibodies called ‘sybodies’. Sybodies can be engineered to target specific protein conformations, labile membrane proteins or protein complexes.

Iwan Zimmermann
Pascal Egloff
Markus A. Seeger
Protocols08 Apr 2020
Nature Protocols

Volume: 15, P: 1707-1741
Weaker connectivity in resting state networks is associated with disinhibited eating in older adults

Anthony Brennan
Lars Marstaller
Hayley A. Young
ResearchOpen Access11 Jan 2022
International Journal of Obesity

Volume: 46, P: 859-865
GFP-based optimization scheme for the overexpression and purification of eukaryotic membrane proteins in Saccharomyces cerevisiae

David Drew
Simon Newstead
So Iwata
Protocols10 Apr 2008
Nature Protocols

Volume: 3, P: 784-798

Search

Wing Interferential Patterns (WIPs) and machine learning, a step toward automatized tsetse (Glossina spp.) identification

Structural basis for antibiotic transport and inhibition in PepT2

Crystal structures of a double-barrelled fluoride ion channel

Cryo-EM structure of the human THIK-1 K2P K⁺ channel reveals a lower Y gate regulated by lipids and anesthetics

Molecular basis for selective uptake and elimination of organic anions in the kidney by OAT1

Extracellular modulation of TREK-2 activity with nanobodies provides insight into the mechanisms of K2P channel regulation

Future opportunities in solute carrier structural biology

Structural basis for proton coupled cystine transport by cystinosin

Molecular basis for redox control by the human cystine/glutamate antiporter system xc⁻

Structural basis of antifolate recognition and transport by PCFT

Molecular basis of nitrate uptake by the plant nitrate transporter NRT1.1

Structural basis for substrate specificity and regulation of nucleotide sugar transporters in the lipid bilayer

Structural basis of Smoothened regulation by its extracellular domains

Structural basis for amino acid transport by the CAT family of SLC7 transporters

Structural basis for triacylglyceride extraction from mycobacterial inner membrane by MFS transporter Rv1410

Insights into L-type heteromeric amino acid transporters

Structural basis of nucleotide sugar transport across the Golgi membrane

A two-___domain elevator mechanism for sodium/proton antiport

The KDEL trafficking receptor exploits pH to tune the strength of an unusual short hydrogen bond

Overcoming barriers to membrane protein structure determination

Generation of synthetic nanobodies against delicate proteins

Weaker connectivity in resting state networks is associated with disinhibited eating in older adults

GFP-based optimization scheme for the overexpression and purification of eukaryotic membrane proteins in Saccharomyces cerevisiae

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