The presence of PHO84 sense and antisense transcripts in single cells is strongly anticorrelated, suggesting a switch-like regulation mechanism. Data from single-molecule fluorescent in situ hybridization experiments provide evidence that the exosome component Rrp6 does not degrade full-length PHO84 antisense transcripts but prevents antisense transcription elongation by favoring early termination.
- Manuele Castelnuovo
- Samir Rahman
- Daniel Zenklusen
