Charge transfer in perovskite oxide heterostructures could break the delicate balance among charge, spin, orbital and lattice order at the interface, producing exotic phenomena that cannot be observed in bulk materials. Here, the authors observe an opposite charge transfer direction in two 3d/5d perovskite oxide heterostructures SrIrO3/LaNiO3 and SrIrO3/NdNiO3, investigate the triggered interfacial orbital and lattice reconstructions, and discuss their manipulation on transport and magnetic characteristics.