Sequencing articles from across Nature Portfolio

The heterogeneity of isoform level m⁶A RNA methylation in single cells is unclear. The authors characterize m⁶A at both single-cell and isoform level through ONT long-read sequencing on single-cell cDNA library with APOBEC1-YTH induced C-to-U mutations. They find the role of m⁶A on surveillance of misprocessed RNAs through CDS-m⁶A decay mechanism.

In this Tools of the Trade article, Jianheng Fox Liu describes CROWN-seq, a method for mapping Am (2′-O-methyladenosine), m⁶Am (N⁶,2′-O-dimethyladenosine) and transcription start sites.

Advances in single-cell multi-omics assays that co-detect two or more modalities enable a new frontier in resolving cellular heterogeneity and gene regulation.

In this Journal Club, Diyendo Massilani recalls two studies by Meyer et al. that reported a mitochondrial genome and nuclear DNA sequences from mid-Ice Age Sima de los Huesos hominins.

In this Tools of the Trade article, Loic Binan explains Perturb-FISH, which measures genetic perturbations and gene expression in situ at high throughput to map gene regulatory networks at cellular and tissue scale.

In this Tools of the Trade article, Nadiya Khyzha describes SLAM-RT&Tag, a method for profiling RNA localization and dynamics within nuclear compartments, such as speckles.