Fig. 5: Knockdown Bub3 together with overexpression of LNC CRYBG3 significantly promotes tumor formation and metastasis of transplanted immortalized human lung epithelial cells. | Oncogene

Fig. 5: Knockdown Bub3 together with overexpression of LNC CRYBG3 significantly promotes tumor formation and metastasis of transplanted immortalized human lung epithelial cells.

From: RETRACTED ARTICLE: The long noncoding RNA CRYBG3 induces aneuploidy by interfering with spindle assembly checkpoint via direct binding with Bub3

Fig. 5

The absolute expression levels of LNC CRYBG3 (A) and Bub3 (B) were determined in 23 clinical lung cancer tissues and four normal lung tissues using quantitative RT-PCR. The data are expressed as the mean ± SEM. C The relationship between the expression of LNC CRYBG3 and Bub3. D Bub3 expression in an established A549 cell line expressing Bub3 shRNA (sh1 and sh2) was detected by western blotting. The experiment was independently repeated three times. E HSAEC1-KT cells stably expressing Bub3 shRNA with or without transfection of LNC CRYBG3 overexpressing lentivirus were inoculated into NOD/SCID mice (n = 5 for each group). Thirty days after the injection, the xenograft tumors were harvested in each group for hematoxylin and eosin staining and other analyses. F The weight of xenograft tumors from different groups. G The expression of Bub3 in xenograft tumors. h In vivo cell metastasis experiment (n = 5 for each group, but four mice of the shBub3 group died). *p < 0.05; **p < 0.01; ***p < 0.001.

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