Fig. 6: LNC CRYBG3 interacts with Bub3 and modulates the interactions of the main components of the mitotic checkpoint complex. | Oncogene

Fig. 6: LNC CRYBG3 interacts with Bub3 and modulates the interactions of the main components of the mitotic checkpoint complex.

From: RETRACTED ARTICLE: The long noncoding RNA CRYBG3 induces aneuploidy by interfering with spindle assembly checkpoint via direct binding with Bub3

Fig. 6

A RNA pulldown analysis was used to determine the Bub3-LNC CRYBG3 interaction in A549 cells. B Histogram of LNC CRYBG3 enrichment after RNA immunoprecipitation (IP) assays. Bub3 antibodies were used. C Immunoprecipitation followed by western blotting was used to detect the interaction of LNC CRYBG3 with MCC components. All experiments were independently repeated at least three times. D Three truncated fragments of LNC CRYBG3 (S1, S2, and S3) were synthesized based on the predicted secondary structure of LNC CRYBG3 and then used for immunoprecipitation to detect the interaction of Bub3 and CDC20. The quantitative data were presented as mean ± SEM. *p < 0.05. E, F The colocalization of Bub3 and CDC20 after overexpression of LNC CRYBG3. G, H The colocalization of Bub3 and CENP-A after the overexpression of LNC CRYBG3. J, K The colocalization of Bub3 and CDC20 after overexpression of truncated body of LNC CRYBG3. I, L The colocalization of Bub3 and CENP-A after the overexpression of truncated body of LNC CRYBG3. Ctrl, A549 cells without transfection; NC, A549 cells transfected with negative control RNA; LNC CRYBG3, A549 cells transfected with LNC CRYBG3 overexpressing adenovirus. All experiments were repeated at least three times independently. The quantitative data were presented as mean ± SEM. *p < 0.05; **p < 0.01.

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