Fig. 5: Cytoprotective effects of ILK are mediated through an ILK-SFK-YAP axis. | Oncogene

Fig. 5: Cytoprotective effects of ILK are mediated through an ILK-SFK-YAP axis.

From: Drug tolerance and persistence to EGFR inhibitor treatment are mediated by an ILK-SFK-YAP signaling axis in lung adenocarcinoma

Fig. 5

a YAP1 expression in HCC4006 Par and OsiR cells (n = 2 experimental replicates each). b Enrichment plot of the YAP expression signature from the Oncogenic Signature gene sets from GSEA comparing HCC4006 OsiR to Par cells. c Western blot of YAP, P-SFK and SRC in HCC4006 Par and OsiR cells. d Representative immunofluorescent confocal imaging of Osi/DMSO-treated HCC4006 shILK cells ± DOX. e Insets of example HCC4006 cells treated with DMSO or Osi. YAP intensity profile across the white line is plotted on the right. f Quantification of nuclear/cytoplasmic YAP intensity in Osi (100 nM) treated HCC4006 shILK (n = 12–14 images in each condition across 3 experimental replicates) and shNS (n = 12 images in each condition across 3 experimental replicates) cells. g Insets of 2 example Osi treated HCC4006 shILK+DOX cells with high and low RFP expression neighbouring each other. YAP intensity profile across the white line is plotted on the right. h Quantification of nuclear/cytoplasmic YAP intensity in Osi/DMSO-treated HCC4006 shILK cells +DOX (n = 12–14 images in each condition across 3 experimental replicates). i Representative western blot of P-SFK and SRC in Osi-treated HCC4006 shILK cells ± DOX. j Quantification of nuclear/cytoplasmic YAP intensity in Osi (50 nM) treated HCC4006 cells ± dasatinib (30 nM DASA) (n = 12 images in each condition across 3 experimental replicates). Statistical analyses were performed with One-way ANOVA with Sidak post-hoc test (*p < 0.05; **p < 0.01; ***p < 0.001; ****p < 0.0001). k Validation of DOX-inducible SRC overexpression HCC4006 shILK p20-GFP/SRC cells ± DOX. l Quantification of GFP or SRC rescue in Osi-treated HCC4006 shILK DTP cells (n = 3 each). Colony counts were normalized to the No DOX condition for each Osi concentration. Multiple paired t-tests with Holm-Sidak correction were used to statistically compare DOX vs No DOX at each Osi concentration (*p < 0.05; **p < 0.01). Error bars represent the mean ± SEM. DOX concentration = 100 ng/mL.

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