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Data availability
The cryo-EM maps and associated atomic coordinate models of the complete RsmZ–A complex have been deposited in the wwPDB OneDep System under EMD accession code EMD-34048 and PDB ID code 7YR7, and the RsmZ–A complex with one missing RsmA homodimer in the wwPDB OneDep System under EMD accession code EMD-34047 and PDB ID code 7YR6.
References
Kang, C.-I. et al. Clin. Infect. Dis. 37, 745–751 (2003).
Hauser, A. R. Nat. Rev. Microbiol. 7, 654–665 (2009).
Høiby, N., Ciofu, O. & Bjarnsholt, T. Future Microbiol. 5, 1663–1674 (2010).
Lee, J. & Zhang, L. Protein Cell 6, 26–41 (2015).
Goodman, A. L. et al. Dev. Cell 7, 745–754 (2004).
Brencic, A. et al. Mol. Microbiol. 73, 434–445 (2009).
Janssen, K. H. et al. J. Bacteriol. 200, e00736–17 (2018).
Papenfort, K. & Vogel, J. Cell Host Microbe 8, 116–127 (2010).
Duss, O. et al. Nature 509, 588–592 (2014).
Duss, O., Michel, E., Diarra dit Konté, N., Schubert, M. & Allain, F. H.-T. Nucleic Acids Res. 42, 5332–5346 (2014).
Morris, E. R. et al. Structure 21, 1659–1671 (2013).
Ma, H., Jia, X., Zhang, K. & Su, Z. Signal. Transduct. Target. Ther. 7, 58 (2022).
Chua, E. Y. D. et al. Annu. Rev. Biochem. 91, 1–32 (2022).
Heeb, S., Blumer, C. & Haas, D. J. Bacteriol. 184, 1046–1056 (2002).
Vakulskas, C. A., Potts, A. H., Babitzke, P., Ahmer, B. M. M. & Romeo, T. Microbiol. Mol. Biol. Rev. 79, 193–224 (2015).
Acknowledgements
Cryo-EM data were collected on Can Cong at SKLB West China Cryo-EM Center and processed at Duyu High Performance Computing Center in Sichuan University. This work was supported by the Ministry of Science and Technology of China (2022YFC2303700 and 2021YFA1301900 to Z.S.), the National Natural Science Foundation of China (32222040 and 32070049 to Z.S., 31970131 to K.Z.), and Sichuan University start-up funding (20822041D4057 to Z.S.); J.M.B. and S.M. were supported by the Polish National Science Center (NCN 2017/26/A/NZ1/01083 and NCN 2021/43/D/NZ1/03360, respectively). Computational resources for SimRNA simulations were provided by the Poznań Supercomputing and Networking Center at the Institute of Bioorganic Chemistry, Polish Academy of Sciences through the Polish Grid Infrastructure (grant: plgsimcryox). Z.M. was supported by R&D program of Guangzhou Laboratory (SRPG22-003).
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Z.S. conceived the project. X.J., Z.P. and G.J. prepared RNAs and proteins. X.J. constructed expression plasmids. X.J., Z.P. and X.L. performed binding experiments. Y.Y., X.Y., X.W. and K.Z. carried out phenotype and qRT-PCR experiments. Y.L. collected cryo-EM data. Y.L., X.J. and B.L. processed cryo-EM data. X.J., B.L., S.M. and J.M.B. built and refined atomic models. L.L. carried out covariation analysis of RsmZ secondary structure and sequence alignment. All authors contributed to the preparation of the manuscript.
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Jia, X., Pan, Z., Yuan, Y. et al. Structural basis of sRNA RsmZ regulation of Pseudomonas aeruginosa virulence. Cell Res 33, 328–330 (2023). https://doi.org/10.1038/s41422-023-00786-3
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DOI: https://doi.org/10.1038/s41422-023-00786-3
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