Fig. 1: Single-cell RNA sequencing analysis of PD-1 expression and distribution in T-cell subsets from the PB, DLT and CLT in AE patients. | Nature Communications

Fig. 1: Single-cell RNA sequencing analysis of PD-1 expression and distribution in T-cell subsets from the PB, DLT and CLT in AE patients.

From: Targeting myeloid-derived suppressor cells promotes antiparasitic T-cell immunity and enhances the efficacy of PD-1 blockade

Fig. 1

a UMAP plot showing the clustering results for 5 major cell types in the high-quality single-cell RNA sequencing data from the PB, DLTs and CLTs of AE patients (n = 4). b Dot plot showing the highly expressed marker genes in each major cell type. The dot size represents the percentage of cells expressing the marker genes in each major cell type, and the dot colour represents the average expression level of the marker genes in each cell type. Blue indicates high expression, while grey indicates low expression. c Stacked histogram showing the percentages of immune cell types among the total immune cells from the PB, DLT and CLT groups. d UMAP plots showing the cellular compositions of PB, DLT and CLT. e Violin plot showing the expression level of PD-1 in the 7 major cell types in the PB, DLT and CLT samples. f UMAP plot showing the distribution of CD4+ T-cell subsets. Each colour represents a CD4+ T-cell subset. The clusters expressing PD-1 are circled. g UMAP plot showing the expression level of PD-1 in 13 subsets of CD4+ T cells. h UMAP plot showing the distribution of CD8+ T-cell subsets. The clusters expressing PD-1 are circled. i UMAP plot showing the expression level of PD-1 in 12 subsets of CD8+ T cells. PB, peripheral blood; DLT, ‘distant’ liver tissue; CLT, ‘close’ liver tissue. Source data are provided as a Source Data file.

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