Fig. 5: PD-1 deficiency does not influence disease progression or T-cell dysfunction in a mouse model of E. multilocularis infection. | Nature Communications

Fig. 5: PD-1 deficiency does not influence disease progression or T-cell dysfunction in a mouse model of E. multilocularis infection.

From: Targeting myeloid-derived suppressor cells promotes antiparasitic T-cell immunity and enhances the efficacy of PD-1 blockade

Fig. 5

a Workflow of the experimental procedure for the PD-1-/- mice and wild type (WT) mice infected with E. multilocularis PSCs. Mice image was drawn by hand with Procreate software. Analysis of liver lesion weight at weeks 2, 12 and 24 post-infection and flow cytometry of T cells in the liver and spleen at week 24 post-infection. b Representative image of metacestode tissue in the livers of wild-type (WT) or PD-1-/- mice at weeks 2, 12, and 24 post-infection. Metacestode tissues are circled in yellow. c Whole liver weight (left) and lesion weight (right) in the livers of WT or PD-1-/- mice at weeks 2 (WT, n = 10; PD-1-/-, n = 12), 12 (WT, n = 10; PD-1-/-, n = 11), and 24 (WT, n = 16; PD-1-/-, n = 10) of persistent infection. d Percentage of CD69+ cells among CD4+ T cells or CD8+ T cells in the livers of WT (n = 5) or PD-1-/- mice (n = 5) at week 24 post-infection. e Percentage of effector memory CD4+ T cells (CD44+CD62L-CD4+ Tem) or CD8+ T cells (CD44+CD62L-CD8+ Tem) in the livers of WT (n = 5) or PD-1-/- mice (n = 5) at week 24 post-infection. f Percentages of CD4+ T cells or CD8+ T cells positively stained for one, two or multiple cytokines (i.e., granzyme B, IFN-γ, TNF-α and IL-2) in the livers of WT (n = 5) or PD-1-/- mice (n = 5) at week 24 post-infection. c–f Data were analysed using two-tailed unpaired Student’s t tests. All data are presented as the mean ± SD from each of at least two independent experiments. n.s., P > 0.05. Source data are provided as a Source Data file.

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