Fig. 9: Liver-infiltrating G-MDSCs upregulate IDO1, which inhibits T-cell function. | Nature Communications

Fig. 9: Liver-infiltrating G-MDSCs upregulate IDO1, which inhibits T-cell function.

From: Targeting myeloid-derived suppressor cells promotes antiparasitic T-cell immunity and enhances the efficacy of PD-1 blockade

Fig. 9

a Representative immunohistochemical staining for CD33, IDO1 or INOS (upper panel 100 ×, lower panel 400×) in liver sections from AE patients (left). Arrowheads indicate CD33+ cells, IDO1+ cells or INOS1+ cells. b, c, d The percentage of positively stained cells was calculated to assess the expression of CD33 (n = 13), IDO1 (n = 15) or INOS (n = 13). e Example of two patients. Patient No.26 with low metabolic activity (PET-CT, SUV = 1.9, left image a) and corresponding staining for CD33 or IDO1 in periphrastic liver tissue (right image, 400 ×). Patient No.63 with high metabolic activity (PET-CT, SUV = 9.4, left image a) and corresponding staining for CD33 or IDO1 in periphrastic liver tissue (right image, 400 ×). Left image (b): Corresponding CT scan; Left Image (c): Corresponding PET/CT fusion image; Left image (d): Corresponding region with radiopharmaceutical distribution in the hepatic lobe, as detected by maximum intensity projection (MIP). Arrows indicate 18F-FDG uptake (hot spots). f Correlations between the percentages of CD33+ cells, IDO1+ cells or INOS+ cells and the FDG-PET uptake value in the perilesional area (n = 14). g Representative images of the immunofluorescence staining for DAPI (blue), CD33 (green), and IDO1 (green) and merged images of liver sections from AE patients (n = 10). Boxed areas show × 200 magnification of histological images. Arrows indicate CD33+ IDO1+ MDSCs. A white line was drawn around the lesion. h Percentage of IDO1+ MDSCs in the livers of wild-type (WT) or PD-1-/- mice at week 24 post-infection (n = 3). i IFN-γ and TNF-α secretion in the supernatant of CD4+ T cells or CD8+ T cells cocultured with G-MDSCs for 48 h in the presence of the control or IDO1 inhibitor (1-MT) was measured by ELISA. The data represent at least two independent experiments. Standard uptake value, SUV. b–d Data were analysed using two-tailed paired Student’s t tests. f Data were analysed using a two-tailed Pearson’s correlation. h Data were analysed using a one-way ANOVA with Tukey’s multiple comparisons test. All data are presented as the mean ± SD. n.s., P > 0.05. Source data are provided as a Source Data file.

Back to article page