Fig. 5: Identification of the initial intermediate for alkane degradation.
From: Thermophilic Hadarchaeota grow on long-chain alkanes in syntrophy with methanogens
a MS analysis of extracts of culture with unlabeled hexadecane showed a peak at m/z = 365.21884 (red), which matches the authentic hexadecyl-CoM standard (blue). b Fragmentation of the isolated m/z range of 365.0–365.4 yields hexadecyl-thiol (C16H33S−, m/z = 257.23082), ethenesulfonate (C2H3SO3−, m/z  =  106.98079) and sulfonate (HSO3−, m/z = 80.96517). These peaks are also produced by hexadecyl-CoM standards. c MS analysis of extracts of culture with 1,2-13C-labeled hexadecane yielded a peak at m/z = 367.22548 (red). d Fragmentation of the isolated m/z range of 367.0–367.4 yielded 1,2-13C-labeled hexadecyl-thiol (C16H33S−, m/z = 259.23739), unlabeled ethenesulfonate (C2H3SO3−, m/z = 106.98084) and sulfonate (HSO3−, m/z = 80.96515). The mass errors for all mass peaks are <5 ppm.
