Fig. 6: Suppression of the hedgehog-like signal increases resistance to mitochondrial stress and pathogen infection but reduces brood size.
From: A germline-to-soma signal triggers an age-related decline of mitochondrial stress response
a, b Survival rate of day 9 wild-type (N2) worms treated with indicated RNAi and exposed to antimycin. RNAi treatment was applied either “whole-life” (starting at L1) or “adult-only” (starting on day 1 of adulthood). c qRT-PCR analysis of UPRmt genes in day 9 N2 adults treated with RNAi and exposed to antimycin. d, e Survival rate of day 5 N2 adults treated with RNAi and exposed to PA14. f Representative fluorescence images of day 5 irg-1p::gfp adults with the indicated treatments. Pathogen infections were done on day 5 of adulthood for 48 h unless otherwise noted. Scale bar, 200 µm. g qRT-PCR analysis of innate immune response genes in day 5 N2 animals exposed to PA14. h Representative fluorescence images of day 5 N2 worms treated with PAO1(GFP). Scale bar, 200 µm. i Quantification of PAO1 or PA14 CFU in day 5 N2 worms. j, k Survival rates of day 9 and day 5 hsp-6p::gfp adults overexpressing piRNAs targeting wrt-5, wrt-6, or a control gene, with antimycin treatment on day 9 and PA14 on day 5. l Brood size of N2 worms treated with RNAi from L1 stage (“whole life”) or day 1 adulthood (“adult-only”). m Brood size of hsp-6p::gfp worms overexpressing piRNAs targeting wrt-5, wrt-6, or a control gene. n Representative fluorescence images of day 5 vit-2::gfp worms treated with RNAi. Scale bar, 200 µm. o qRT-PCR analysis of the vit family genes in day 1 N2 adults treated with RNAi. Error bars indicate mean ± SD. n represents the number of independent experiments for panels (c, g, i, o), and the number of worms for panels (i, m). p values were assessed using a two-tailed t test for panels (c, g, i, l, m, o), and using the Log-rank (Mantel–Cox) test for panels (a, b, d, e, j, k). Source data are provided as a Source Data file.
