Fig. 1: Overview of the synthetic anaerobic-aerobic consortium.

a Overall illustration of the process; one strain co-consumes xylose and glucose and produces xylitol and acetic acid, while the other strain co-consumes glucose and acetic acid and produces IBA. b Overview of knockouts in the synthetic anaerobic xylitol producing strain. c Overview of knockouts in the aerobic acetic acid auxotrophic co-metabolizing IBA producing strain. d Illustration of the anhydrotetracycline inducible CRISPRi-enabled metabolic switch to repress the expression of cytochrome BD-I.