Fig. 5: Interactions between SaSir2 and SaHerA. | Nature Communications

Fig. 5: Interactions between SaSir2 and SaHerA.

From: Mechanistic basis for the allosteric activation of NADase activity in the Sir2-HerA antiphage defense system

Fig. 5

a Surface and cartoon representation of SaSir2-HerA, showing the minimal SaSir2-HerA unit. b, c Specific interactions between SaSir2 and SaHerA; the residues involved in their interactions are shown as sticks. d Deletion of the N-terminal residues of Sir2 and HerA, encompassing residues 1–34 and 1–35, simultaneously abolished the NADase activity of Sir2-HerA. Data were presented as mean ± SD in histograms from three replicates. P-values were determined by paired two-tailed t-tests. e In vitro pull-down assays of SaSir2 with an N-terminal 1–34 deletion and HerA with an N-terminal 1–35 deletion, His-tagged HerA was used as bait, Sir2 was used as a pray. Source data are provided as a Source Data file.

Back to article page