Fig. 4: Detection of α-tubulin and kinetochore proteins in metaphase chromosomes of L. sylvatica. | Nature Communications

Fig. 4: Detection of α-tubulin and kinetochore proteins in metaphase chromosomes of L. sylvatica.

From: Repeat-based holocentromeres of the woodrush Luzula sylvatica reveal insights into the evolutionary transition to holocentricity

Fig. 4

a KNL1 (orange) and CENH3 (green) proteins co-localize and show a holocentric distribution (n = 15). b KNL1 protein localizes specifically to the centromere surface, where microtubules (gray) bind (n = 15). c Co-detection of KNL1, Lusy1 repeats (purple), and α-tubulin (n = 15). It is noteworthy that at this stage, some microtubule ends already colocalize with KNL1 proteins (indicated by arrowheads, see Supplementary Figs. 7 and 8). Maximum intensity projections of 3D-SIM image stacks.

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