Fig. 4: H2S release from the click-and-release reaction between sydnthione 1b and DIBAC.
From: Sydnthiones are versatile bioorthogonal hydrogen sulfide donors
1b (100 μM) with different reagents in 20% DMSO/PBS (pH 7.4, 10 mM) at 37 °C during 30 min. (1) 1b only; (2) carbonic anhydrase (CA) (25 μg mL−1); (3) DIBAC (100 μM), CA (25 μg mL−1); (4) DIBAC (500 μM), CA (25 μg mL−1); (5) DIBAC (1.0 mM), CA (25 μg mL−1); (6) DIBAC (1.5 mM), CA (25 μg mL−1); (7) DIBAC (2.0 mM), CA (25 μg mL−1); (8) DIBAC (2.5 mM), CA (25 μg mL−1); (9) DIBAC (1.0 mM); (10) DIBAC (1.0 mM), CA (25 μg mL−1), acetazolamide (AAA) (2.5 μM). Experiments were conducted in triplicate and results are expressed as mean ± SD (n = 3 independent samples). Source data are provided as a Source Data file.
