Fig. 8: Mutually orthogonal release of H2S and Dox in H9c2 cells based on mutual orthogonality between sydnthione‒DIBAC and tetrazine‒MOHO bioorthogonal cycloaddition pairs.
From: Sydnthiones are versatile bioorthogonal hydrogen sulfide donors
a Mutual orthogonality between 1b‒DIBAC and Tz-Dox‒MOHO reaction pairs. b Images of orthogonal release of H2S and Dox in H9c2 cells. I: H9c2 cells were incubated with 1b (30 μM), Tz-Dox (30 μM), and DCI-CHO-DNP (10 μM) for 3.5 h at 37 °C. II-IV: H9c2 cells were incubated with 1b (30 μM), Tz-Dox (30 μM), and DCI-CHO-DNP (10 μM) for 30 min, and then treated with either DIBAC (300 μM, II) or MOHO (300 μM, III) or both (IV) for 3 h at 37 °C. Scale bar = 10 μm. The experiment was independently repeated three times with similar results.
