Fig. 9: Effects on cell viability through the release of H2S from sydnthione 1b in Dox-treated H9c2 cells.
From: Sydnthiones are versatile bioorthogonal hydrogen sulfide donors
a H9c2 cells were treated with 1b (0–12.5 μM) at 37 °C for 30 min, then incubated with 10 eq. DIBAC for 6 h. Cells were then treated with DMSO for additional 24 h. b H9c2 cells were treated with 1b (0–12.5 μM) at 37 °C for 30 min, then incubated with 10 eq. DIBAC and 10 μM Dox for 24 h. c, d H9c2 cells were treated with 1b (0–12.5 μM) at 37 °C for 30 min, then incubated with 10 eq. DIBAC for c 3 h or d 6 h. Cells were then treated with 10 μM Dox for additional 24 h. Data are presented as mean ± SD (n = 3 independent cell pellets). Statistical differences were analyzed by Student’s two-sided t-test between Ctrl and indicated groups. ns, not significant. Source data are provided as a Source Data file.
