Fig. 2: MERFISH maps non-parenchymal cells within the healthy liver.
From: Spatial transcriptomics of healthy and fibrotic human liver at single-cell resolution
a, b Spatial distribution of all cells defined by MERFISH within the architecture of the healthy liver for tissue from two different donors, donor 1 (a) and donor 2 (b). Scale bars: 1000 μm. c, e, g Spatial distribution of macrophage (c), HSC (e), and cholangiocyte and EC (g) populations mapped in the same tissue section as a. Scale bars: 1000 μm. d, f, h Spatial distribution of cells in the same tissue section as (a) colored by the normalized expression of the indicated marker genes. CD74 and CD5L identify macrophages (d), PDGFRB and LAMB1 identify HSCs (f), KRT7 identifies cholangiocytes, and DNAS1L3 identifies ECs (h). Scale bars: 1000 μm. i Dot plot quantifying expression of genes that identify non-parenchymal cells in the liver. The size of the dot represents the percentage of cells expressing a specific gene, and the color intensity indicates mean expression.
