Fig. 3: OsHox12 interacts with CTB5 and positively regulates cold tolerance at the booting stage.
From: Natural variation of CTB5 confers cold adaptation in plateau japonica rice
a, b Relative expression levels of OsHox12 in different tissues (a) and at different time points during cold treatment (b). Data represent means ± SD (n = 3 biological replicates). c Interactions of OsHox12 with CTB5KM/Tow in yeast. EV, the empty pGADT7 vector. d–f Split-luciferase complementation (d), co-immunoprecipitation (e), and bimolecular fluorescence complementation (f) assays showing the interaction between OsHox12 and CTB5. OsCam1-1 in d and f was used as a negative control. Total proteins from rice protoplasts co-expressing Flag-CTB5 with OsHox12-Myc were immunoprecipitated with Myc beads and detected with anti-Flag antibody in e. Scale bar in f = 5 μm. g Relative expression levels of OsHox12 in OsHox12 overexpression lines. Data represent means ± SD (n = 3 biological replicates). h, i Seed-setting rates of Towada and OsHox12 overexpression lines under cold stress in a high-altitude area (CS-HAA) conditions (n = 17/14/14 plants) (h) and in a phytotron (CS-PT) conditions (n = 12/12/12 plants) (i). j Comparison of panicles between Towada and OsHox12 overexpression lines under CS-HAA. Scale bar = 2 cm. k, l Seed-setting rates of NILCTB5 and OsHox12 knockout lines under CS-HAA (n = 17/14/13 plants) (k) and CS-PT (n = 10/10/10 plants) (l). m Seed-setting rates of NILCTB5 and double mutants of OsHox12 and CTB5 under CS-PT (n = 10 plants). n Comparison of panicles between NILCTB5 and OsHox12 knockout lines under CS-HAA. Scale bar = 2 cm. In (c–f), a representative experiment from at least two independent experiments is shown. In (g–i, k–m), different lowercase letters indicate statistically significant differences at P = 0.05 by one-way ANOVA with Duncan’s multiple-range test, and data represent means ± SD. Source data are provided as a Source Data file.
