Fig. 2: Inhibition mechanism of Cas12a cleavage activity by heparin sodium.
From: Tunable control of Cas12 activity promotes universal and fast one-pot nucleic acid detection
a Schematic representation of the three potential stages of Cas12a activity inhibited by heparin sodium. b, c Impact of heparin sodium on Cas12a cleavage activity at three distinct stages when crRNA targets f3l (b) or b6r (c). d The binding affinity of crRNA for Cas12a in the presence of heparin sodium using EMSA. e ΔG of crRNA, heparin sodium, and S-free heparin with LbCas12a. f Root Mean Square Deviation (RMSD) of LbCas12a in different simulation systems. g–k, Electrostatic potential analysis between Cas12a and crRNA (g); Cas12a and heparin (h); Cas12a, crRNA, and heparin (i); Cas12a and S-free heparin (j); Cas12a, crRNA, and S-free heparin (k). Data are shown as mean ± s.d. for n  =  3 biologically independent samples.
