Fig. 3: Heparin sodium-based one-pot Cas12a detection method.

a, b Schematic of the one-pot detection method, where RPA amplification and Cas12a-mediated fluorescence detection occur simultaneously within a single tube. b–d Characterization of the LoD of the RPA-Cas12a one-pot detection method without (b) and with 40 µg/mL heparin sodium (c) and the qPCR (d), targeting the f3l gene at concentrations of 0, 0.1, 1, 10, 100, 1000, 10000 aM. e–g LoD characterization of the RPA-Cas12a one-pot method targeting b6r without (e) and with the 40 µg/mL heparin sodium (f) and the qPCR (g). h, i At a target concentration of 10 fM, with Cas12a-crRNA (100 nM) targeting f3l (h) or b6r (i), the content of RPA amplification products at various times were analyzed in agarose gels. Samples without heparin sodium or Cas12a during the RPA amplification process were used as the controls. Data are shown as mean ± s.d. for n  =  3 biologically independent samples. The schematics shown in Fig. 3a were created by figdraw.com.