Fig. 1: CERT inhibition with HPA-12 reduces the viability and promotes the apoptosis of AML cell lines.
From: Targeting ceramide transfer protein sensitizes AML to FLT3 inhibitors via a GRP78-ATF6-CHOP axis
a Western blot of the CERT protein in healthy donors and AML cell lines (HL-60, THP-1, KG-1α, MV4-11, and Molm13). HD, bone marrow nucleated cells from healthy donors. b Viability of OCI-AML3, HL-60, THP-1, Kasumi-1, KG-1α, MV4-11, and Molm13 cells treated with HPA-12 at the indicated concentrations for 48 h. Effects of CERT knockdown (KD) on the proliferation of MV4-11 (c) and Molm13 (d) cells. n = 5 independent experiments (c), and n = 3 independent experiments (d). KD, knockdown. e–h Molm13 cells were treated with increasing concentrations of HPA-12 for 48 h. Cells treated with the indicated concentrations of HPA-12 were subjected to an EdU assay (e, f) and Annexin V/PI staining (g, h). EdU-positive cells were identified by ImageJ. Scale bar, 50 μM. n = 3 independent experiments. The data are presented as the means ± SDs, and differences were compared by 2-tailed Student’s t tests. Multiple groups were analyzed with one-way ANOVA. ns: not significant, *P < 0.05; **P < 0.01; ***P < 0.001; ****P < 0.0001. Source data are provided as a source data file.
