Fig. 2: Characterization of resistant tumor cells against combination therapy. | Nature Communications

Fig. 2: Characterization of resistant tumor cells against combination therapy.

From: Resistance to anti-LAG-3 plus anti-PD-1 therapy in head and neck cancer is mediated by Sox9+ tumor cells interaction with Fpr1+ neutrophils

Fig. 2

A The UMAP plot shows five epithelial subtypes in distinct colors, with bar graphs indicating their quantities. B The UMAP plot (left) shows the distributions of control, resistant, and sensitive groups, with cell proportions (center) and subtype compositions (right). C Heatmap of top 10 signature genes for three groups. D CellChat circle plot shows differences in intercellular communication number and strength between two groups. E The UMAP plot shows 11 immune cell subtypes, with bar graphs indicating their numbers. F The UMAP plot shows 11 immune subtypes across two groups. G CellChat analysis compares epithelial-immune information flow in Resi and Sens groups, with relative (left) and absolute (right) flow shown. H Scatter plot of ANNEXIN pathway signaling in cell clusters from CellChat analysis. Bubble plots from CellChat (I) and CellPhoneDB (J) show epithelial subtype communication with Neu1 receptors and ligands. P values were calculated using a permutation test, assessing the significance of cell-cell communication by comparing observed mean expression with a null distribution generated by random permutations (I). P values were calculated using a permutation test, which generates a null distribution by randomly shuffling cell labels to determine the specificity of interactions (J). P values are one-sided and exact. K Violin plot (left) and FeaturePlot (right) show Anxa1 expression in epithelial cells across groups. The box plot elements are defined as described in the Statistics and Reproducibility section. Each group includes cells from 3 mice. P values were calculated one-way ANOVA. L Violin plot showing Anxa1 expression across epithelial cell subtypes. The box plot elements are defined as described in the Statistics and Reproducibility section. Each group includes cells from 9 mice. P values were calculated one-way ANOVA. M Representative IHC staining and score of Anxa1 in HNSCC. n = 6 control, n = 6 resistant, n = 8 sensitive mice. Scale bar = 65 μm. Data shown as as mean ± SD. P values were calculated one-way ANOVA with Tukey’s multiple comparisons test. Source data and exact p values are provided as a Source Data file.

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