Fig. 4: Model of stalled nascent chain extraction by the RQC complex.
From: Mechanism of nascent chain removal by the ribosome-associated quality control complex
a Model of the RQC complex including the predicted model of E2 (Rad6) and its accompanying donor ubiquitin. b Close up view of Rqc1CTD, acceptor ubiquitin, and Ltn1RING fitted in cryo-EM reconstruction density with the predicted orientation of E2 bound to donor ubiquitin. Key interaction residues between E2, donor ubiquitin (G76), and acceptor ubiquitin (K48) are labeled. The positions of other lysines on the acceptor ubiquitin are also labeled for spatial context. K11 and K27 are also inaccessible for ubiquitylation but are not labeled due to obscured views. Reconstruction density is low-pass filtered for clarity. c Schematic of nascent chain extension, ubiquitylation, and extraction by the RQC complex.
