Fig. 6: In vivo live imaging of NHPs implanted with RPE cell suspensions. | npj Regenerative Medicine

Fig. 6: In vivo live imaging of NHPs implanted with RPE cell suspensions.

From: Allogeneic RPE cell suspension manufactured at scale demonstrating preclinical safety and efficacy led to IND approval

Fig. 6

Cases #1-OD and #1-OS, color fundus pictures show clear view of the posterior chamber (Ai–Bi), and optically clear in the vitreous cavity. Optic disc and retinal vascular architecture appear normal (Aii–Bii). The white lines (Aiii–Biii) indicate the position at which SD-OCT images (Aiv–Biv) taken. ERM was observed on the retinal surface under H&E staining (Av–Bv). C Case #2-OS, the white arrows indicating the ‘cells lump’ at 1 week, 1 month and 3 months. The white doted lines indicate the position of OCT line scan. D Immunohistochemical analysis within injection sites of case #2-OS. Multi-layer transplanted cells were observed at all three locations (positive for Tra-1-85, negative for RPE65, with pigmentation) on top of the host RPE cells (negative for Tra-1-85, positive for RPE65). Scale bars indicate 200 μm in A(iv), B(iv) and (C); 100 μm in A(v) and B(v), 50 μm in (D).

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