Extended Data Fig. 8: ECM ligands and mechanics dictate CF activation, mechanosensitive, and senescence phenotypes. | Nature Materials

Extended Data Fig. 8: ECM ligands and mechanics dictate CF activation, mechanosensitive, and senescence phenotypes.

From: Hybrid hydrogel–extracellular matrix scaffolds identify biochemical and mechanical signatures of cardiac ageing

Extended Data Fig. 8

ICC images shown in Fig. 4j and k were quantified for α-SMA intensity and stress fiber assembly for young CFs (a, c) and aged CFs (b, d). ICC images shown in Fig. 6l and m were quantified for p53 integrated intensity and mean intensity for young CFs (e, g) and aged CFs (f, h). ICC images of young (i) and aged CFs (j) on DECIPHER samples stained for nucleus, F-actin, YAP (i, j), and TIMP3 (k, l). SoftY and StiffA TIMP3 images are also shown in Fig. 6k. One-way ANOVA with Fisher’s preplanned multiple comparison (each pair with one variable, ECM or stiffness) was used for (a-h), N = 3 biological replicates for each condition. Scale bar: 50 µm (i, j), 100 µm (k, l).

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