Fig. 5: MW-TMPs promote cytotoxic T/NK cell expansion.
a, The umap plot of NK and T cells. b, Heat map of normalized expression of NK and T cell classical genes among six clusters. TFs, Transcription factors. c, The gene signature scores of memory/differentiation, activation/inhibitory, effector and transcription factor expression in CD8-c1, CD8-c2 and CD8-c3. d, The developmental trajectory analysis of CD8+ T cells. e, The distribution of CD8-subtypes among groups treated with respective intervention. f, The cytotoxic and exhausted gene signature scores among PBS, MW-TMP and UV-TMP groups (n = 3). g, Ratios of CD8+ T, CD4+ T cells in tumours via FCM (n = 5). h, Representative immunofluorescence graphs of tumours, spleens and lymph nodes (n = 5). Green, CD8; red, CD4. Scale bars, 20 μm. i, The proportions of Treg cells in tumours (n = 5). j,k, The proportions of Th1 (j) and Th2 (k) cells in tumours (n = 5). l,m, Cytotoxic CD8+ T cells in tumours secrete higher percentages of Gzmb (l) and IFNγ (m) with the treatment of MW-TMPs (n = 5). n, The umap plot of cytotoxic effector genes coloured by cluster in the NK group. o, The cytotoxic and exhausted gene signatures for the two NK subclusters (n = 3). p, The proportion of NK subgroups in the untreated and treated groups (n = 5). q, The proportions of total NK cells and activated subgroups (IFNγ+ NK) in tumours (n = 5). The data in f, g, i–m, o and q are shown in analytic plots as mean ± s.d., and ordinary one-way ANOVA was used for data in f, g, i–m, o and q. The exact P value was noted.
