Extended Data Fig. 2: Biological activity of CPM chemical analogues in vitro.
From: A condensate-hardening drug blocks RSV replication in vivo
a, c, e, g, Potency of compounds to inhibit SHH signalling was tested in Gli-dependent luciferase-reporter-expressing NIH3T3 cells. Data are expressed as the percentage ± s.d. of the DMSO control. Data are representative of two experiments performed in triplicate. b, d, f, h, Potency of compounds to inhibit RSV replication was tested in RSV–Luc-infected HEp-2 cells. Luciferase activity was measured at 24 h after infection and expressed as the percentage ± s.d. of the DMSO control. The cytotoxicity of compounds was measured in parallel in either HEp-2 or NIH3T3 reporter cells after treatment for 24 h (data not shown) or 72 h. IC50 and CC50 values were calculated with GraphPad software. Data shown are representative of two (b, f) or three (d, h) experiments performed in triplicate.
