Extended Data Fig. 4: Disorganization of IB and IBAG architecture in RSV–M2-1–mGFP-infected and compound-treated cells.
From: A condensate-hardening drug blocks RSV replication in vivo
HEp-2 cells were infected with RSV–M2-1–mGFP for 24 h and then treated for 1 h with A3E and CPM at the indicated concentrations and stained to detect poly(A) RNA and N protein as described in the Methods. a, Representative images from two independent experiments are shown. Poly(A) RNA (red), N (white), M2-1–mGFP (green) and nuclei (blue) were visualized. A merged image of red, green and blue channels is shown (merge). Scale bars, 10 μm. b, The presence of IBAGs within IBs was quantified and indicated as IBAG (+) when visible and IBAG (−) when absent (described in the Methods) for approximately 50 infected cells per condition from two independent experiments.
