Fig. 5: GET identifies a cell-type-specific TF–TF interaction affected by a cancer-associated germline variant.
From: A foundation model of transcription across human cell types
a, pLDDT plot for PAX5, showing three mutational hotspots (V26G, P80R and G183S/A/V) and a frameshift hotspot46. DBD, DNA-binding ___domain. b, B cell-specific motif interactions of PAX/2. PAX5 is the most highly expressed TF with a PAX/2 motif. The colour scheme follows that of Fig. 4a. c, AlphaFold3-predicted multimer structure of PAX5 IDR and NR2C2 NR ___domain showing contacts around G183 (B, back; f, front). The blue–yellow surface at the back represents hydrophilicity–hydrophobicity, respectively. Blue–red strands in the front show low–high prediction confidence. d, Detection of NCOR1, NRIP1, NR3C1 and NR2C2 PAX5-interacting proteins in streptavidin-enriched eluates (enrichment) from PAX5-WT, PAX5 G183S and RHOA WT-BioID-expressing B-ALL REH cell lines and in total protein lysates (1% input) using proximity labelling assays. Three independent experiments with similar results. A representative experiment is shown. GAPDH was used as a loading control in the same gel. Raw gel images are in Supplementary Fig. 2. e, Quantification of PAX5–NR2C2 interaction in the streptavidin immunoprecipitation shown in d. Data are presented as mean values with s.d. as well as total NR2C2-normalized values.
