Extended Data Fig. 8: Manipulation of BLA or CeA terminals in DMS is neither rewarding or aversive.
From: A dual-pathway architecture for stress to disrupt agency and promote habit
(a) Following training and testing (Fig. 3h–n) mice receive a real-time place preference test in which 1 side of a 2-chamber apparatus was paired with optogenetic inhibition of BLA axons and terminals in the DMS. Average percent time spent in light-paired chamber across 2, 10-min sessions (one with light paired with each side). 2-sided t-test: t(19) = 0.65, P = 0.52, 95% CI −0.04 − 0.08. eYFP N = 10 (5 male), Arch N = 11 (5 male) mice. Males = closed circles, Females = open circles. Data presented as mean +/− SEM. (b-c) Following training and testing mice receive a real-time place preference test in which 1 side of a 2-chamber apparatus was paired with optogenetic stimulation of DMS-projecting CeA neurons. (b) Average percent time spent in light paired chamber across 2, 10-min sessions (one with light paired with each side) in handled control subjects. 2-sided t-test: t(21) = 1.75, P = 0.10, 95% CI −0.79 − 9.06. eYFP N = 17 (9 male), ChR2 N = 6 (3 male) mice. (c) Average percent time spent in light paired chamber across 2, 10-min sessions (one with light paired with each side) in subjects with a prior once/daily stress for 14 d. 2-sided t-test: t(16) = 0.52, P = 0.61, 95% CI −3.74 − 6.17. eYFP N = 8 (4 male), ChR2 N = 10 (6 male) mice. Males = closed circles, Females = open circles. Data presented as mean +/− SEM.
