Extended Data Fig. 7: Experimental evolution of the MuLTEE ancestors and t1000 isolates with selection against larger size.
From: Genome duplication in a long-term multicellularity evolution experiment
a, Experimental setup. We evolved PM/PA t0 and PM/PA1-5 t1000 isolates, each with four replicate populations (A, B, C, D), under selection against larger size for 70 days (~500 generations) by growing them on agar in 24-well plates with daily dilution. b, Distributions of cluster radius (weighted by cluster volume) in the ancestral (“anc”) and evolved populations (on average, n = 406 clusters measured per population). Vertical thick solid line, biomass-weighted mean cluster radius of each population. Vertical dashed line, biomass-weighted mean cluster radius of PM/PA t0. Color codes for the evolved ploidy in each t0 population and in each t1000 population are the same as those in Fig. 3h,i, respectively, and the ancestral populations are colored in gray.
