Extended Data Fig. 8: Chemogenetic and optogenetic manipulation of vHipp D1 and D2 neurons during anxiety-related testing – controls and additional metrics.
From: Dopamine D1–D2 signalling in hippocampus arbitrates approach and avoidance
Effects of CNO itself (n = 10 CNO, n = 10 vehicle male mice) on a, OA exploration time (t-test: t14.65 = −0.061 p = 0.9523); b, total number of open arm entries (left; t-test: t17.99 = −0.53 p = 0.6045) and average OA exploration bout length (right; t-test: t17.26 = −0.057 p = 0.9548). c, Experimental schematic. Data are obtained from the same mice as in Fig. 4a–c (n = 12 D1-mCherry, n = 8 D1-hM3Dq, n = 11 D2-mCherry, n = 12 D2-hM3Dq male mice). d, Total number of OA entries (left; t-test: t8.16 = 0.73 p = 0.4857) and average OA exploration bout length (right; t-test: t16.42 = 1.05 p = 0.3095) for D1-Cre mice. e, Total number of OA entries (left; t-test: t20.68 = −2.16 p = 0.0423) and average OA exploration bout length (right; t-test: t14.63 = −2.18 p = 0.0463) for D2-Cre mice. f, Experimental schematic. Data are obtained from the same mice as in Fig. 4d–f (n = 12 D1-mCherry, n = 11 D1-hM4Di, n = 12 D2-mCherry, n = 12 D2-hM4Di male mice). g, Total number of OA entries (left; t-test: t20.94 = 0.64 p = 0.5284) and average OA exploration bout length (right; t-test: t20.60 = −1.89 p = 0.0723) for D1-Cre mice. h, Total number of OA entries (left; t-test: t21.68 = 1.55 p = 0.1354) and average OA exploration bout length (right; t-test: t21.93 = 1.80 p = 0.0858) for D2-Cre mice. i, Experimental schematic. (n = 11 D1-mCherry, n = 11 D1-hM3Dq, n = 11 D2-mCherry, n = 12 D2-hM3Dq male mice for OFT, and n = 11 D1-mCherry, n = 11 D1-hM4Di, n = 12 D2-mCherry, n = 12 D2-hM4Di for NSF). j, Total locomotor activity in OFT for D1-Cre (left; t-test: t13.25 = −2.52 p = 0.0253) and D2-Cre (right; t-test: t20.99 = 0.08 p = 0.9374) mice. k, Time spent in the OFT center zone for D1-Cre (left; t-test: t18.84 = 2.6052 p = 0.0175) and D2-Cre (right; t-test: t19.62 = −0.63 p = 0.5379) mice. l, Latency to the first feeding bout during NSF testing for D1-Cre (left; t-test: t18.31 = 0.51 p = 0.6171) and D2-Cre (right; t-test: t21.82 = −2.32 p = 0.0303) mice. m, Experimental schematic. Data are obtained from the same mice as in Fig. 4g–i (n = 10 D1-EYFP, n = 9 D1-ChR2, n = 11 D2-EYFP, n = 10 D2-ChR2 male mice). n, Total number of OA entries (left; LMM-ANOVA: stimulation zone F1,17 = 0.67 p = 0.4232, virus F1,17 = 2.63 p = 0.1234, stimulation zone x virus F1,17 = 2.55 p = 0.1284; followed by FDR-adjusted post-hoc tests) and OA exploration bout length (right; LMM-ANOVA: stimulation zone F1,17 = 8.88 p = 0.0084, virus F1,17 = 0.58 p = 0.4580, stimulation zone x virus F1,17 = 5.8529 p = 0.0271; followed by FDR-adjusted post-hoc tests) for D1-Cre mice. o, Total number of open arm entries (left; LMM-ANOVA: stimulation zone F1,19 = 8.70 p = 0.0082, virus F1,19 = 7.94 p = 0.0110, stimulation zone x virus F1,19 = 0.036 p = 0.8526; followed by FDR-adjusted post-hoc tests) and OA exploration bout length (right; LMM-ANOVA: stimulation zone F1,19 = 2.62 p = 0.1221, virus F1,19 = 6.1645 p = 0.0225, stimulation zone x virus F1,19 = 9.55 p = 0.0060; followed by FDR-adjusted post-hoc tests) for D2-Cre mice. Data represented as mean ± sem.
