Extended Data Fig. 2: Immunocompetent mouse models of leptomeningeal metastasis.
From: Interferon-γ orchestrates leptomeningeal anti-tumour response
(a) Overview of cancer cells lines used and generated in this study. (b) Principal component analysis (PCA) of in vitro transcriptome of Parental (grey, n = 3), LeptoM (purple, n = 3), and BrM1 (orange, n = 3) LLC cells. Retrieved from NCBI GEO GSE83132. (c) Kaplan-Meier plot showing survival of C57Bl/6-Tyrc-2 animals overexpressing Egfp in the leptomeninges after delivery of LLC LeptoM cells into cisterna magna (related to Fig. 3c). Representative brain tissue sections stained with H&E showing colonization of leptomeninges after intracardiac delivery of LLC LeptoM cells (scale bar = 100 μm). mOS - median overall survival. (d) Principal component analysis (PCA) of in vitro transcriptome of Parental (grey, n = 3) and newly established LeptoM (purple, n = 4), and BrM2 (orange, n = 3) B16 cells. (e) Kaplan-Meier plot showing survival of C57Bl/6 animals overexpressing Egfp in the leptomeninges after delivery of B16 LeptoM cells into cisterna magna (related to Extended Data Fig. 9f). Representative brain tissue sections stained with H&E showing colonization of leptomeninges after intracardiac delivery of B16 LeptoM cells (scale bar = 100 μm). mOS - median overall survival. (f) Principal component analysis (PCA) of in vitro transcriptome of Parental (grey, n = 3) and newly established LeptoM (purple, n = 3), and BrM2 (orange, n = 3) Yumm5.2 cells. (g) Kaplan-Meier plot showing survival of C57Bl/6 and C57Bl/6-Tyrc-2 animals overexpressing Egfp in the leptomeninges after delivery of Yumm5.2 LeptoM cells into cisterna magna (related to Extended Data Fig. 9l). Representative brain tissue sections stained with H&E showing colonization of leptomeninges after intracardiac delivery of Yumm5.2 LeptoM cells (scale bar = 100 μm). mOS - median overall survival. (h) Principal component analysis (PCA) of in vitro transcriptome of Parental (grey, n = 3) and newly established LeptoM (purple, n = 5), and BrM2 (orange, n = 3) E0771 cells. (i) Kaplan-Meier plot showing survival of C57Bl/6-Tyrc-2 animals overexpressing Egfp in the leptomeninges after delivery of E0771 LeptoM cells into cisterna magna (related to Extended Data Fig. 9d). Representative brain tissue sections stained with H&E showing colonization of leptomeninges after intracardiac delivery of E0771 LeptoM cells (scale bar = 100 μm). mOS - median overall survival. (j) Principal component analysis (PCA) of in vitro transcriptome of Parental (grey, n = 3) and newly established LeptoM (purple, n = 3), and BrM2 (orange, n = 3) EMT6 cells. (k) Kaplan-Meier plot showing survival of BALB/c animals overexpressing Egfp in the leptomeninges after delivery of EMT6 LeptoM cells into cisterna magna (related to Extended Data Fig. 9h). Representative brain tissue sections stained with H&E showing colonization of leptomeninges after intracardiac delivery of EMT6 LeptoM cells (scale bar = 100 μm). mOS - median overall survival. (l) Principal component analysis (PCA) of in vitro transcriptome of Parental (grey, n = 3) and newly established LeptoM (purple, n = 3), and BrM2 (orange, n = 3) 4T1 cells. (m) Kaplan-Meier plot showing survival of BALB/c animals overexpressing Egfp in the leptomeninges after delivery of 4T1 LeptoM cells into cisterna magna (related to Extended Data Fig. 9j). Representative brain tissue sections stained with H&E showing colonization of leptomeninges after intracardiac delivery of 4T1 LeptoM cells (scale bar = 100 μm). mOS - median overall survival.
