Extended Data Fig. 3: Interaction between leptomeningeal metastasis and systemic immunity.
From: Interferon-γ orchestrates leptomeningeal anti-tumour response
(a) In vivo radiance of subcutaneous LLC LeptoM tumours two weeks after injection of leptomeningeal tumour from the same subline. Quantification shows extracranial radiance at four weeks. (b) In vivo radiance of leptomeningeal LLC LeptoM tumours in the presence of subcutaneous tumour from the same subline. Extracranial tumours were injected two weeks before intracisternal implantation, and quantification shows cranial radiance after two weeks. Related to panel N. (c) In vivo radiance of mammary E0771 LeptoM tumour two weeks after injection of leptomeningeal tumour from the same subline. Quantification shows extracranial radiance at four weeks. (d) In vivo radiance of leptomeningeal E0771 LeptoM tumours in the presence of mammary tumour from the same subline. Extracranial tumours were injected two weeks before intracisternal implantation, and quantification shows cranial radiance after two weeks. Related to panel P. (e) Proportion of major leptomeningeal immune cell types in control and LLC LeptoM-bearing mice. Cells were injected subcutaneously and/or intracisternally. Related to panel N. (f) Proportion of major leptomeningeal immune cell types in control and LLC LeptoM-bearing mice. Cells were injected into lungs and/or intracisternally. (g) Proportion of major leptomeningeal immune cell types in control and E0771 LeptoM-bearing mice. Cells injected into mammary fat pad and/or intracisternally. Related to panel P. (h) Proportion of major leptomeningeal and lung immune cell types in control and E0771 LeptoM-bearing mice. Cells were injected intracardially (multiple t-tests; column numbers for comparison are shown below the group sizes, B – B cells, T – T cells, MM – monocytes & macrophages, Neu – neutrophils, NK – NK cells, cDC – conventional dendritic cells, pDC – plasmacytoid dendritic cells).
