Extended Data Fig. 7: Characterization of AV and tumor organoid cultures.
a, Left, Pearson correlation (r2 and two-sided p-value) of ATAC-seq profiles of uncultured AV cells versus their derivative organoid culture. Right, chromatin accessibility tracks of representative genes. b, Representative IHC staining of ELF5, JUN, and pSer73-JUN in organoid cultures of normal AV cells and tumor cells. Scale bars = 200 μm. Results are representative of two experiments. c, Bar graphs (mean ± s.e.m.) showing RT-qPCR quantification of expression levels of Wnt10a and Wnt target genes, Axin2 and Tcf7, in organoid cultures of AV cells (n = 3 WT, 7 HET) and BPP tumor cells (n = 2). Each dot represents an independent organoid line, with its gene expression level averaged across 2-8 independent technical replicates. d, Bar graphs (mean ± s.e.m.) showing RT-qPCR quantification of expression levels of dominant-negative mutant Jun-DN upon doxycycline induction (2 μg/mL) in Brca1-HET AV organoids and the corresponding levels of Wnt10a and other Gp2-associated genes. P-values were determined by two-sided unpaired t-test (n = 3 technical replicates). Results are representative of two independent experiments. Similar plots for Jun-DN and Wnt10a are shown Fig. 5f and are included here for completeness. e, Bar graphs (mean ± s.e.m.) showing the effects of doxycycline-induced (0, 0.02, 0.2, and 2 μg/mL) expression of Jun on the levels of Wnt10a and other Gp2-associated genes in AV organoids as measured by RT-qPCR. P-values were determined by two-sided unpaired t-test (n = 3 technical replicates). Results are representative of three independent experiments. Similar plots for Jun and Wnt10a are shown Fig. 5e and are included here for completeness. f, Bar graphs (mean ± s.e.m.) showing RT-qPCR quantification of expression levels of dominant-negative mutant Jun-DN upon doxycycline induction (2 μg/mL) in tumor organoids and the corresponding levels of Wnt10a and other Gp2-associated genes. P-values were determined by two-sided unpaired t-test (n = 3 technical replicates). Results are representative of two independent experiments.
