Extended Data Fig. 3: Trp63-expressing and Nkx3.1-expressing cell labeling. | Nature Genetics

Extended Data Fig. 3: Trp63-expressing and Nkx3.1-expressing cell labeling.

From: JAK/STAT signaling maintains an intermediate cell population during prostate basal cell fate determination

Extended Data Fig. 3

a,b, Construction strategy of T-TLR (Trp63-DreER;R26-TLR) mice (a) and N-TLR (Nkx3.1-CreER;R26-TLR) mice (b) for Trp63+ and Nkx3.1+ cell labeling, respectively. c, Schedule of tamoxifen injection and analysis in T-TLR and N-TLR mouse prostates. d-f, Immunofluorescence staining of Trp63, Nkx3.1, Ck8, ZsGreen and tdTomato in T-TLR (d and e) and N-TLR (f) mouse prostates. g, Percentage of Trp63+ (n = 12,757), Trp63− (n = 0), Ck8+ (n = 0), and Ck8− (n = 15,300) cells among ZsGreen+ cells in T-TLR mice (n = 4). h, Percentage of Nkx3.1+ (n = 14,438) and Nkx3.1− (n = 0) cells among tdTomato+ cells in N-TLR mice (n = 4). i, Immunofluorescence staining of Trp63, Nkx3.1, Ck8 and tdTomato in N-TLR mouse prostates. j, Percentage of Ck8+ luminal cells and Trp63+ basal cells among tdTomato+ cells (n = 27,195) in N-TLR mice (n = 4). k, Percentage of Nkx3.1+ cells (n = 2,649) and Nkx3.1− cells (n = 0) among tdTomato+Trp63+ cells in N-TLR mice (n = 4). Scale bars, 50 μm. Data are shown as the mean ± SD.

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