Extended Data Fig. 5: AMP Formation from Tri17 assay and proposed reaction mechanism.
From: Biosynthesis of triacsin featuring an N-hydroxytriazene pharmacophore
LC-UV detection of AMP at 260 nm from an assay containing 15, nitrite, ATP, and Tri17. Controls lacking enzyme or nitrite result in undetectable amounts of AMP, while the production of AMP was slightly increased in the presence of 15. ATP is proposed to activate nitrite to form a nitrite-AMP intermediate that undergoes a subsequent nucleophilic attack by 15 and tautomerization to yield 1. At least three independent replicates were performed for each assay, and representative results are shown.
