Extended Data Fig. 4: In vitro analysis of Tri14, a putative thioesterase.
From: Biosynthesis of triacsin featuring an N-hydroxytriazene pharmacophore

a) EICs demonstrating hydrolysis of lauroyl-S-Tri20 catalyzed by Tri14, resulting in production of lauric acid when compared to an authentic standard. Tri14 was shown to be Tri20 specific and imparted no activity on lauroyl-S-Tri30. The calculated mass for lauric acid: m/z = 199.1701 ([M-H]−) is used for each trace. b) EIC demonstrating inability of Tri14 to hydrolyze hexanoyl-S-Tri20, thus suggesting that Tri14 activity is chain length specific. The calculated mass for hexanoic acid: m/z = 115.0764 ([M-H]−) is used for each trace. A 10-ppm mass error tolerance was used for each trace. At least three independent replicates were performed for each assay, and representative results are shown.